Lavender top(EDTA), pink (K2EDTA), blue (Na Citrate) or yellow (Acid Citrate Dextrose) blood tubeġ-7 days, contact Molecular Diagnostics (40) for quicker resultĬYP2C19, 2C19, Cytochrome P450 2C19, P450 2C19 Genotyping, Plavix, clopidogrel, antiulcer drugs, antidepressant drugs, antiseizure drugsĬharacteristics: CYP2C19 is involved in the metabolism of many drugs such as clopidogrel (Plavix), benzodiazepines, proton pump inhibitors, and voriconazole. (2009) Quantitative PCR: A quality control assay for estimation of viable virus content in live attenuated goat pox vaccine.Plavix Pharmacogenetics - CYP2C19, 8 Variants Kallesh D, Hosamani M, Balamurugan V, Bhanuprakash V, Yadav V, et al. Gustafsson RKL, Engdahl EE, Fogdell-Hahn A (2012) Development and validation of a Q-PCR based TCID(50) method for human herpesvirus 6. Jonsson N, Gullberg M, Lindberg AM (2009) Real-time polymerase chain reaction as a rapid and efficient alternative to estimation of picornavirus titers by tissue culture infectious dose 50% or plaque forming units. Journal of Clinical Microbiology 51: 1528–1533. Popowitch EB, O'Neill SS, Miller MB (2013) Comparison of the Biofire FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVPv1, and Luminex xTAG RVP Fast Multiplex Assays for Detection of Respiratory Viruses. Journal of Clinical Microbiology 50: 3458–3465. Pierce VM, Hodinka RL (2012) Comparison of the GenMark Diagnostics eSensor Respiratory Viral Panel to Real-Time PCR for Detection of Respiratory Viruses in Children. The relationship between TCID50/mL concentrations and the corresponding copy number related to various ATCC cultures is also reported. Broader detection of influenza A/H3 viruses was demonstrated by the GenMark eSensor RVP. The multiplex PCR platform, the GenMark eSensor RVP, demonstrated improved analytical sensitivity for detecting influenza A/H3 viruses, influenza B virus, human parainfluenza virus 2, and human rhinovirus (1.6-94.8 copies/μL, 0.20-1.98 logs). Largest differences in LOD were demonstrated for assays targeting adenovirus group E, respiratory syncytial virus subtype A, and a generic assay for all influenza A viruses regardless of subtype (319.4-1280.8 copies/μL, 2.50-3.11 log difference). Assays targeting influenza A/H3N2, influenza A/H1N1pdm09, influenza B, and human parainfluenza 1 and 2 were most comparable (1.2-8.4 copies/μL, <1 log difference). Analytical sensitivity between the two methods varied from 1.2-1280.8 copies/μL (0.08-3.11 log differences) for all 12 assays compared. The GenMark eSensor RVP LODs were obtained by converting the TCID50/mL concentrations reported in the package insert to copies/μL using qPCR. LODs were measured for each singleplex real-time PCR assay and expressed as the lowest copy number detected 95-100% of the time, depending on the assay. In this experiment, the lower limit of detection (LOD) of singleplex real-time PCR assays targeting respiratory viruses is compared to an equivalent panel on a multiplex PCR platform, the GenMark eSensor RVP. However, multiplexed assays must be well optimized to retain or improve upon the analytic sensitivity of their singleplex counterparts. Multiplex PCR methods are attractive to clinical laboratories wanting to broaden their detection of respiratory viral pathogens in clinical specimens.
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